Thermodynamics and Kinetics of Freezing
We use infrared and Raman spectroscopy to determine the kinetic and thermodynamic phenomena in the freeze-concentrated liquid during freezing, vitrification, and thawing. This gives us unique access to molecular level changes proteins, and enzymes experience when they interact with their environments at low temperatures and crowding. Of specific interest is their interections with the ice interface. Some of our recent papers on this topic are:
[Langmuir, 2018, DOI: 10.1021/acs.langmuir.8b02982]
[J Phys Chem B, 2013, 117 (26), 7889]
[RSC Adv, 2017, 7, 43611]
[Pharm Res, 2017, 34, 462]
[Pharm Res, 2016, 33, 1413]
Room Temperature Storage of Archival Serum Samples
Many cancer biomarkers in the bood get damaged during long term storage when kept at cryogenic temperatures due to stresses associated with ice formation and low temperatures. The aim of this research is to develop isothermal vitrification methods to ensure that the archival serum samples are stably stored at room temperature for a long time.
We have developed a lyoprotectant matrix that is used for stabilizing archival serum samples. Serum is pipetted into the wells filled with the lyoprotectant matrix, and dried overnight in a vacuum chamber, which vitrifes the sample making it stable during room temperature storage. The matrix is an electrospun non-woven fibrous material electrospun from a patent pending lyoprotectant solution. The capillary forces help adsorb the serum into the matrix, and dissolve to uniformly load the serum with the lyoprotectants, avoiding clumping.
The results of the initial validation studies we have conducted have recently been published [Scientific Reports, 6, 24186, 2016];
We are currently in the process of collecting longer term storage results after making some modifications to the existing matrix. Stay tuned!